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KMID : 0613820060160071207
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Journal of Life Science
2006 Volume.16 No. 7 p.1207 ~ p.1213
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Tumor Necrosis Factor-α Promotes Osteogenesis of Human Bone Marrowderived Mesenchymal Stem Cells through JNK-dependent Pathway
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Kim Mi-Ra
Song Hae-Young
Kim Jae-Ho
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Abstract
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Tumor necrosis factor-α (TNF-α) has been implicated in skeletal diseases by promoting bone loss in inflammatory bone diseases. In the present study, we examined the effects of TNF-α on osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). TNF-α dose-dependently promoted matrix mineralization of hBMSCs with a maximal stimulation at 2 ng/ml. TNFα increased expression of alkaline phosphatase, which plays a crucial role for the matrix deposition. The TNF-α-stimulated osteoblastic differentiation was not affected by NFκB inhibitors, BAY and SN50. However, a JNK-specific inhibitor, SP600125 completely abolished the TNF-α-stimulated matrix mineralization and expression of alkaline phosphatase. These results suggest that TNF-α enhances osteoblastic differentiation of hBMSCs through JNK-dependent pathway.
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KEYWORD
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Mesenchymal stem cells, TNF-alpha, differentiation, JNK, bone
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